BPC157 / TB500
(the Woolverine stack)

What is the Wolverine Stack?

The "Wolverine stack" is a popular, yet largely experimental, combination of two synthetic peptides—BPC-157and TB-500. Named after the Marvel comic character, it is widely discussed in performance, biohacking, and athletic circles for its purported ability to rapidly accelerate tissue repair and recovery.

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What is BPC157?

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BPC-157: Derived from a protein naturally found in the stomach, it is studied for its role in healing muscles, tendons, ligaments, and the gut lining by reducing localized inflammation and promoting blood vessel formation.

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What is TB500?

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A synthetic version of the protein thymosin beta-4, it is investigated for its ability to migrate cells to injury sites, improve actin dynamics, and aid in body-wide tissue regeneration.

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How does it work?

The "Wolverine stack" combines the peptides BPC-157 and TB-500 to accelerate tissue repair and recovery from injuries. It works through two complementary, overlapping mechanisms: 

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BPC-157 (Body Protection Compound-157): Works locally. Derived from a protective protein in stomach acid, it promotes angiogenesis (new blood vessel growth), reduces inflammation, and stimulates collagen production to rebuild tendons, ligaments, and muscle. 

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TB-500 (Thymosin Beta-4): Works systemically. A synthetic version of a natural protein that aids in tissue regeneration by boosting cell migration and repairing structural cells. 

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What do I get?

The stack is typically supplied as a freeze-dried (lyophilised) powder in a small 10mg vial made up of 5mg of BPC157 and 5,g of TB500. Before use, it must be reconstituted (Mixed) with  one millilitre of bacteriostatic water. This involves slowly adding the water for injection to the vial and gently mixing until the powder is fully dissolved. Injections of 1.0mL (10 units on 1mL syringe) will give you 10 injections of 1mg of the Woolverine stack. 

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Why would I consider using it?

People use it in biohacking, fitness, and regenerative health circles to try to accelerate the healing of muscles, tendons, and ligaments, reduce inflammation, and speed up overall soft-tissue recovery.

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How do I prepare for use?

To reconstitute the peptide you will need additional items:

Shringes - 1ml Insulin - EG: https://amzn.eu/d/06IXjKNG

Bacterioustatic water - EG:  https://amzn.eu/d/0aBduzbc

Alcohol wipes - EG: https://amzn.eu/d/073r0cc1

Steps to Reconstitute Peptides.

Reconstituting peptides is a straightforward process that ensures accurate dosing and preserves the peptide's integrity. Follow these steps carefully:

• Clean the Vial Tops:

  • Swab the rubber stoppers on both the peptide vial and the bacteriostatic water vial with alcohol wipes. Allow them to air dry.

• Draw the Solvent:

  • Using the insulin syringe, draw the desired amount of bacteriostatic water.

• Inject the Water:

  • Insert the needle into the peptide vial at a slight angle. Slowly inject the bacteriostatic water down the inside wall of the vial to minimise bubbling.

• Dissolve the Peptide:

  • Gently swirl the vial until the powder is fully dissolved. Avoid shaking the vial, as this can damage the peptide structure.

You can learn how to properly reconstitute peptides here: https://youtu.be/n8vk8DfwRHg

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How do I inject?

Preparing for Injection

• Wash Hands: Clean your hands thoroughly with soap and water or an alcohol-based hand sanitiser.

• Select Injection Site: Common sites include:

  • Abdomen (avoid the area around the navel)

  • Upper outer thighs

  • Upper outer arms (if someone else is administering)

Administering the Injection

• Clean the Injection Site: Use an alcohol pad to clean the area where you will inject.

• Draw the Dose: Draw the prescribed dose into the syringe.

• Pinch the Skin: Pinch a fold of skin at the injection site.

• Insert the Needle: Insert the needle at a 45-degree angle into the fatty tissue just beneath the skin.

• Inject the Peptide: Push the plunger to deliver the medication.

Post-Injection Care

• Dispose of the Needle: Place the used syringe in a sharps container immediately.

• Monitor the Injection Site: Look for any signs of irritation or adverse reactions.

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Important Tips

• Rotate Injection Sites: To prevent tissue damage, always use a new site at least 1-2 inches away from the previous injection.

How do I store my peptide?

• Reconstituted peptides have a shelve life of approximately 28days and should be stored at a temperature of 2-8 degrees Celsius.

• Store reconstituted vials in the back of the fridge, not the door (which has temperature swings).

• Minimise time out of the fridge: Draw your dose quickly (under 2 minutes) and return the vial immediately.

• Protect from light: UV and visible light degrade peptides .

Are there any side effects?

The Woolverine stack is an experimantal peptide combination taken for tissue repair and inflammation. Some individuals may experience mild irritation, redness, or allergic reactions. It's advisable to perform a patch test before full application to minimise potential side effects.

• Redness at the application site

• Itching or irritation

• Temporary tenderness

Resourses:

Peptide Mixing, Dosing and Injecting Safely Video:

https://youtu.be/3VmMvS43Hz0?si=OxonXvV1OzKZzg0a

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Equipment:

Shringes - 1ml Insulin - EG: https://amzn.eu/d/06IXjKNG

Bacterioustatic water - EG:  https://amzn.eu/d/0aBduzbc

Alcohol wipes - EG: https://amzn.eu/d/073r0cc1

IMPORTANT NOTICE